A bright field spheroid counter,
allowing reagent-free follow-up and
morphological observation
Description
Research field
-
3D culture based-drug discovery and regenerative medicine research
-
Non-invasive research that researchers cannot fix/stain cultured cells
High throughput
-
54 second scan time per 1 micro well plate
-
90 seconds for spheroids counting and quantifying area, diameter
and pseudo volume for each spheroid
Entry model for 3D culture analysis
-
Entry model and optimized for 3D culture analysis and bright-field imaging
Features
Cell3iMager neo
(Single-plate)
CC-3000
Cell3iMager
(4-plate)
CC-5000
Scan
1 min
Image
analysis
0.5 min
Export
＆ analyze
Easy workflow
1. High-throughput bright field scanners for 3D spheroids
-
Cell3iMagers are bright field scanners designed specifically to simplify and accelerate quantitative and qualitative measurement of 3D spheroids.
-
This unique LED-based imaging system allows the user to identify and measure single or multiple spheroids per well in a microplate rapidly and automatically.
-
The companion 3D optimized analysis software allows faster image capture, measurement and data analysis
2. Z-stucking of 3D cellular structures
-
Cell3iMager has a focus composite function.
-
Merge stacking images of each Z-direction, for example gel culture or soft agar medium.
-
Measure/quantify mass of cells spread in the Z-direction at once
3. Compatible with various 3D culture microplates
Clear-wall U-shape bottom
Sumitomo Bakelite,
Thermo Fisher Scientific, NOF
Clear-wall flat bottom
CORNING, IWAKI
Hanging-drop plate
InSphero
Black-wall flat bottom
SCIVAX
Soft agar
Stained 2D cultured cell
Cancer cell proliferation in soft agar medium
Neurospheres in suspension culture
Intestinal epithelial cells organoids
Spheres cultured in U-shaped well
-
Compatible with a wide variety of 3D culture microplates
4. Intelligent 3D software
-
Supports basic (single-time point) and time-course dose-response analysis, as well as a multi-planar (focus bracketing) scanning option to use with hydrogel-embedded spheroids
-
30+ adjustable parameters for optimizing object identification, live/dead discrimination (e.g., bubbles, fibers, debris), and compensate for edge of well shadows and distortion as well as reduction of background noise
-
Identify and measure microtissues in single spheroid per well or multiple spheroid per well formats